Improving Salt Stress Tolerance of Pineapple cv. Queen Using Cobalt In vitro

This investigation aimed to focus on how cobalt can avoid the damage caused by salinity stress (NaCl) on Pineapple cv. Queen in vitro. Multiplicated pineapple explants (10 – 12 mm) were subjected for eight weeks to different NaCl conc. (0, 65, 135 or 200 mM) half of them were treated firstly with 5 mg/L Cobalt sulphate. Vegetative growth parameters (no.of shoots, no. of leaves, and shoot length/explant), mineral composition (N, P, K, Na, Cl, Fe, Mn, Zn, Cu and cobalt), proline and protein content were determined. Molecular characterization using PCR based RAPD was carried out to describe the genetic differences resulted from the studied treatments, (salinity and salinity combined with cobalt sulfate). Results show that, pineapple explants growth under salt stress wasn’t prohibited completely specially below 135 mM of NaCl, but it affected negatively with the highest salt stress 200 mM of NaCl. Explants treated with cobalt before subjected to salinity scored the highest significant percentage of vegetative growth characteristics compared with those untreated. Explants treated firstly with cobalt resulted in a significantly decrease of Na+ and Cl-. Cobalt has a positive effect on Macro and Micronutrients, proline and protein content. A total of 34 DNA fragments varying from 186-1456 (bp) were amplified, of which 16 were polymorphic and seven observed as a unique markers that revealed 64.03% polymorphism.

Glanular closure during the tubularized incised plate procedure: a prospective randomized study

Hypospadias is reported in 1:200 live births. The introduction of the tubularized incised plate [TIP] procedure has revolutionized the treatment of hypospadias. The method of glanular closure during the TIP procedure, whether in single or double layers, has not been evaluated in the literature. To evaluate the optimal technique of glanular closure during the TIP repair of the distal hypospadias. Sixty-six infants and children with distal penile hypospadias were treated primarily with the TIP procedure. Age ranged between 9 months and 4 years with a mean age of 20.8 months at time of repair. Patients were randomly subdivided into two equal groups. In group A, the glans was repaired in a single layer, while in group B, the glans was closed in two layers. All cases were subjected to the same protocol of urethral stenting, penile bandage, and catheter removal by the third postoperative day. Both groups were followed up and compared with regard to the results of TIP repair and the incidence of complications. In group A, two cases developed glanular disruption versus none in group B. this was found statistically significant [p<0.05]. No statistical difference was found in between groups with regard to meatal stenosis, fistula formation, or in the overall cosmetic and functional outcomes. Medium-term follow up showed excellent results in 82.9% while good results were obtained in 17.2%. On the mid-term follow up, the TIP procedure continues to prove itself as the gold standard in treatment of the distal hypospadias. The double-layered glanuloplasty seems to hold an improved morbidity profile, with significantly lower glanular disruption rate, when compared to the single layered repair. Large-numbered studies are still needed to provide further evidence for the superiority of the double-layered glanuloplasty.

Anion gap estimation in the diagnosis and prognosis of pregnancy-induced hypertension

To identify the role of the anion gap estimation in the diagnosis and prognosis of pregnancy-induced hypertension. This Cohort study included 224 pregnant women that have attented the antenatal clinic. These comprise 101 women who had developed pregnancy-induced hypertension and 123 healthy pregnant women. Serum electrolytes including sodium, potassium and chloride, blood gases including bicarbonate level, serum albumin and 24 hour-proteins in urine were estimated. Anion gap was then calculated and consequently correlated to the clinical and laboratory data in both groups. Anion gap was significantly decreased in the hypertensive pregnant women than the healthy ones [P < 0.001]. This observation was more severe in the proteinuric hypertensive women than the non-proteinuric women [P< 0.01]. The value of the anion gap was negatively correlated to the severity of the disease. Anion gap depression was significantly increased with the development of placental abruption, intrauterine growth restriction, intrauterine fetal death as well as prematurity [P < 0.05] but this correlation was not observed on the cases of fetal distress. Anion gap estimation could be a reliable test as a diagnostic adjunct in women with pregnancy-induced hypertension as well as a good prognostic test for the development of the complications

Comparative study of hypertonic saline versus normal saline nasal wash in pediatric chronic sinusitis.

The objective of this study was to compare the effect of nasal wash with hypertonic saline [HS] [3.5%] versus normal saline [NS] [0.9%] on CS. Thirty patients with chronic sinusitis [CS] aged 3-16 years were studied. They were randomly divided into two treatment groups matched in age and severity of the disease. Each individual was treated with either HS or NS for four weeks. All patients were evaluated by two clinical scores [cough and nasal secretions/postnasal drip, PND] and by a radiology score at the beginning of the study and after four weeks. The results showed that the HS group was significantly improved in all scores [average +/- SD], e.g. cough score from 3.6 +/- 0.51 to 1.6 +/- 0.74, nasal secretion/PND score from 2.86 +/- 0.35 to 1.6 +/- 0.74 and radiology score from 8.06 +/- 1.28 to 2.66 +/- 1.04. The NS treatment group showed a significant improvement only in the PND score [from 2.66 +/- 0.49 to 1.53 +/- 0.83], but no significant change was reported in both cough score [from 3.53 +/- 0.52 to 3.33 +/- 0.49] and the radiology score [from 8.13 +/- 1.25 to 7.86 +/- 0.91]. It was concluded that HS nasal wash is an efficient treatment of CS

Estimation of postmortem interval based on the third component of complement [C3] cleavage in the blood of albino rats

To estimate postmortem interval [PMI], the spontaneous conversion of the native third component of complement [C3] to its derived fragments in whole blood was studied by immunodiffusion method. Thirty adult albino rats with average weight ranging from 380-400 g were used in this experiment. Immediately after death by decapitation, the collected blood samples were incubated at 37°C, 23°C and 14°C. Sera from each sample were removed every 12 h at 37°C until 60 h, every day at 23°C until the fifth day, and every two days at 14°C until tenth days. The percent age of cleavage of the third component of complement [C3] were assayed. Statistical analysis of the results showed that the incubation of whole blood at a higher temperature led to a faster conversion of beta IC [native C3] to beta IA [C], the cleavage% age at 37°C was about 3 times as that at 14°C [360 mg%. 258 mg% respectively]. Also, there was a significant positive correlation between the percent ages of C3 cleavage and PMI, at the temperatures of [37, 23, 14°C], the correlation coefficients [r] were 01981, m 01.99436, 0.9549 respectively. Therefore, the percent age of C3 cleavage could be used as a marker for estimation of the time passed since death, and this unusual approach could be a step towards the development of an accurate method for determining PMI

use of collagen implant [zenoderm] in the treatment of oro-antral communications and fistulae

Although several methods of surgical repair of oro-antral fistulae have been described, only few have received wide acceptance. The most commonly used methods of closure rely on the mobilization of soft tissue flaps and require a degree of surgical expertise that may not be available at the time of creation of an oro-antral communication. In this study, a collagen implant material [Zenoderm], in a thin sheet form was used for closure of OAC [eight cases] and OAF [two cases]. A simple surgical procedure was used. Closure was achieved successfully in all the cases involved in this study

management of oral hemangiomas by cryosurgery electrosurgery

Hemangiomas can present difficult management problems to the oral and maxillofacial surgeon when their removal is indicated. The present study was under taken to evaluate clinically the applications of cryosurgery and the use of electrosurgery in the management of soft tissue hemangiomas. The clinical material consisted of 12 cases of soft tissue hemangiomas of intra oral region. Amoils B.M.S. 40 cryoapparatus with a tonsillar probe was used with all cases. A high rate of cure was achieved by careful attention to adequate freezing of the tissues and to follow up after treatment at regular intervals; favorable results and high acceptablility strongly support more frequent use of this surgical technique in the management of soft tissue hemangiomas

Lingual approach for removal of impacted mandibular third molars: incidence of postoperative sequelae

This technique described in this study relies on the development of a mucoperiosteal flap lingual to the third molar. Forty-one patients of impacted mandibular third molar were employed in this study. The result of the study were discussed